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Analytical Chemistry : A Toolkit for Scientists and Laboratory Technicians
Analytical Chemistry : A Toolkit for Scientists and Laboratory Technicians
Autore Ham Bryan M
Edizione [2nd ed.]
Pubbl/distr/stampa Newark : , : John Wiley & Sons, Incorporated, , 2024
Descrizione fisica 1 online resource (689 pages)
Disciplina 543
Altri autori (Persone) MaHamAihui
ISBN 9781119894476
9781119894452
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto Cover -- Title Page -- Copyright -- Dedication -- Contents -- About the Authors -- Preface -- Acknowledgments -- About the Companion Website -- Chapter 1 Chemist and Technician in The Analytical Laboratory -- 1.1 Introduction-The Analytical Chemist and Technician -- 1.2 Today's Laboratory Chemist and Technician -- 1.2.1 Computers in the Laboratory -- 1.2.2 Laboratory Information Management Systems (LIMS) -- 1.3 ChemTech-The Chemist And Technician Toolkit Companion -- 1.3.1 Introduction to ChemTech -- 1.3.2 Opening ChemTech -- 1.3.3 Online ChemTech -- 1.4 Chapter Layout -- 1.4.1 Glassware, Chemicals, and Safety -- 1.4.2 Basic Math and Statistics -- 1.4.3 Graphing and Plotting -- 1.4.4 Making Laboratory Solutions -- 1.4.5 Titrimetric Analysis -- 1.4.6 Electrochemistry -- 1.4.7 Laboratory Information Management System (or Software) LIMS -- 1.4.8 Instrumental Analyses-Spectroscopy -- 1.4.9 Instrumental Analyses-Chromatography -- 1.4.10 Instrumental Analyses-Mass Spectrometry -- 1.4.11 Small Molecule and Macromolecule Analysis -- 1.5 USERS OF ChemTech -- Chapter 2 Introduction to the Analytical Laboratory -- 2.1 Introduction to the Laboratory -- 2.1.1 The Scientific Method -- 2.2 Laboratory Glassware -- 2.2.1 Volumetric Flasks -- 2.2.2 Beakers and Erlenmeyer Flasks -- 2.2.3 Graduated Cylinders -- 2.2.4 Pipettes -- 2.2.5 Evaporating Dishes -- 2.2.6 Flames and Furnaces in the Laboratory -- 2.2.7 Laboratory Fume Hoods -- 2.2.8 Drying Ovens -- 2.2.9 Balances -- 2.2.10 Refrigerators and Freezers -- 2.2.11 Test Tubes -- 2.2.12 Soxhlet Extractions -- 2.2.13 Vacuum Pumps -- 2.3 Conclusion -- Chapter 3 Laboratory Safety -- 3.1 Introduction -- 3.2 Proper Personal Protection and Appropriate Attire -- 3.2.1 Proper Eye Protection -- 3.2.2 Proper Laboratory Coats -- 3.3 Proper Shoes and Pants -- 3.4 Laboratory Gloves -- 3.4.1 Natural Rubber (Latex) -- 3.4.2 Nitrile.
3.4.3 Neoprene -- 3.4.4 Butyl -- 3.4.5 Polyvinyl Chloride (PVC) -- 3.4.6 Polyvinyl Alcohol (PVA) -- 3.4.7 Viton -- 3.4.8 Silver Shield/4H -- 3.5 General Rules to Use Gloves -- 3.6 Safety Data Sheet (SDS) -- 3.7 Emergency Eyewash and Face Wash Stations -- 3.8 Emergency Safety Showers -- 3.9 Fire Extinguishers -- 3.9.1 Types of Fires -- 3.10 Clothing Fire in The Laboratory -- 3.11 Spill Cleanup Kits -- 3.12 Chemicals and Solvents -- 3.13 First Aid Kits -- 3.14 Gasses and Cylinders -- 3.15 Sharps Containers and Broken Glass Boxes -- 3.16 Occupational Safety and Health Administration (OSHA) -- Chapter 4 Basic Mathematics in The Laboratory -- 4.1 Introduction to Basic Math -- 4.2 Units and Metric System -- 4.2.1 Introduction to the Metric System -- 4.2.2 Units of the Metric System -- 4.2.3 Converting the SI Units -- 4.3 Significant Figures -- 4.3.1 Significant Figure Rules -- 4.4 Scientific Calculators -- 4.4.1 Example Calculator -- 4.4.2 Window's Calculator -- 4.5 ChemTech Conversion Tool -- 4.5.1 Using the Conversion Tool -- 4.5.2 Closing the Conversion Tool -- 4.6 Chapter Key Concepts -- 4.7 Chapter Problems -- Chapter 5 Analytical Data Treatment (Statistics) -- 5.1 Errors in the Laboratory -- 5.1.1 Systematic Errors -- 5.1.2 Random Errors -- 5.2 Expressing Absolute and Relative Errors -- 5.3 Precision -- 5.3.1 Precision Versus Accuracy -- 5.4 The Normal Distribution Curve -- 5.4.1 Central Tendency of Data -- 5.5 Precision of Experimental Data -- 5.5.1 The Range -- 5.5.2 The Average Deviation -- 5.5.3 The Standard Deviation -- 5.6 Normal Distribution Curve of a Sample -- 5.7 ChemTech Statistical Calculations -- 5.7.1 Introduction to ChemTech Statistics -- 5.7.2 ChemTech Chapter 5 -- Student's Distribution T Test For Confidence Limits -- 5.8.1 Accuracy -- 5.8.2 The Student's t Test -- 5.8.3 Calculating the Student's t Value -- 5.8.4 Probability Level.
5.8.5 Online ChemTech Program for Statistics -- 5.8.6 Sulfate Concentration Confidence Limits -- 5.8.7 Sulfate t Distribution Curve -- 5.8.8 Determining Types of Error -- 5.8.9 Determining Error in Methodology -- 5.9 Tests of Significance -- 5.9.1 Difference in Means -- 5.9.2 Null Hypothesis -- 5.10 Treatment of Data Outliers -- 5.10.1 The Q Test -- 5.10.2 The Tn Test -- 5.11 Chapter Key Concepts -- 5.12 Chapter Problems -- Chapter 6 Plotting and Graphing -- 6.1 Introduction to Graphing -- 6.1.1 The Invention of the Graph -- 6.1.2 Importance of Graphing -- 6.2 Graph Construction -- 6.2.1 Axis and Quadrants -- 6.3 Rectangular Cartesian Coordinate System -- 6.4 Curve Fitting -- 6.5 Redrawn Graph Example -- 6.6 Graphs of Equations -- 6.6.1 Introduction -- 6.6.2 Copper Sulfate Data -- 6.6.3 Plotting the Data -- 6.6.4 Best Fit Line -- 6.6.5 Point‐Slope Equation of a Line -- 6.6.6 Finding the Slope (m) -- 6.6.7 Finding the y‐Intercept (b) -- 6.6.8 Solving for x -- 6.6.9 Estimating the Slope and Intercept -- 6.6.10 Deriving the Equation from the Slope and Intercept -- 6.7 Least‐Squares Method -- 6.7.1 Plotting Data with Scatter -- 6.7.2 Linear Regression -- 6.7.3 Curve Fitting the Data -- 6.8 Computer‐Generated Curves -- 6.8.1 Using ChemTech to Plot Data -- 6.8.2 Entering the Data -- 6.8.3 Plotting the Data -- 6.8.4 Linear Regression of the Data -- 6.8.5 Adding the Best‐Fit Line -- 6.8.6 Entering a Large Set of Data -- 6.9 Calculating Concentrations -- 6.10 Nonlinear Curve Fitting -- 6.11 Chapter Key Concepts -- 6.12 Chapter Problems -- Chapter 7 Using Microsoft Excel® in the Laboratory -- 7.1 Introduction to Excel® -- 7.2 Opening Excel in ChemTech -- 7.3 The Excel Spreadsheet -- 7.3.1 Spreadsheet Menus and Quick Access Toolbars -- 7.4 Graphing in Excel -- 7.4.1 Making Column Headings -- 7.4.2 Entering Data into Columns -- 7.4.3 Saving the Spreadsheet.
7.4.4 Constructing the Graph -- 7.4.5 Inserting a Chart -- 7.4.6 The Chart Source Data -- 7.4.7 Chart Options -- 7.5 Complex Charting in Excel -- 7.5.1 Calcium Atomic Absorption (AAS) Data -- 7.5.2 Entering Ca Data into Spreadsheet -- 7.5.3 Average and Standard Deviation -- 7.5.4 Using the Formula Search Function -- 7.5.5 Inserting the Chart -- 7.5.6 Formatting the Chart -- 7.6 Statistical Analysis using Excel -- 7.6.1 Open and Save Excel StatExp.xlsx -- 7.6.2 Sulfate Data -- 7.6.3 Excel Confidence Function -- 7.6.4 Excel Student's t Test -- 7.6.5 Excel Tools Data Analysis -- Chapter 8 Making Laboratory Solutions -- 8.1 Introduction -- 8.2 Laboratory Reagent Fundamentals -- 8.3 The Periodic Table -- 8.3.1 Periodic Table Descriptive Windows -- 8.4 Calculating Formula Weights -- 8.5 Calculating The Mole -- 8.6 Molecular Weight Calculator -- 8.7 Expressing Concentration -- 8.7.1 Formal (F) Solutions -- 8.7.2 Molal (m) Solutions -- 8.7.3 Molar (M) Solutions -- 8.7.4 Normal (N) Solutions -- 8.8 The Parts PER (PP) Notation -- 8.9 Computer‐Based Solution Calculations -- 8.9.1 Computer‐Based Concentration Calculation-Molarity I -- 8.9.2 Computer‐Based Concentration Calculation-Molarity II -- 8.9.3 Computer‐Based Concentration Calculation-Normality I -- 8.9.4 Computer‐Based Concentration Calculation-Normality II -- 8.10 Reactions in Solution -- 8.11 Chapter Key Concepts -- 8.12 Chapter Problems -- Chapter 9 Acid-Base Theory and Buffer Solutions -- 9.1 Introduction -- 9.2 Acids and Bases in Everyday Life -- 9.3 The Litmus Test -- 9.4 Early Acid-Base Descriptions -- 9.5 Bronsted-Lowry Definition -- 9.6 The Equilibrium Constant -- 9.7 The Acid Ionization Constant -- 9.8 Calculating the Hydrogen Ion Concentration -- 9.9 The Base Ionization Constant -- 9.9.1 OH− Ion Concentration Example -- 9.9.2 Percent Ionization Example -- 9.10 Ion Product for Water.
9.11 The Solubility Product Constant (KSP) -- 9.11.1 Solubility of Silver(I) Thiocyanate -- 9.11.2 Solubility of Lithium Carbonate -- 9.12 The pH of a Solution -- 9.13 Measuring The pH -- 9.13.1 The Glass Electrode -- 9.14 Buffered Solutions-Description and Preparing -- 9.14.1 Le Chatelier's Principle -- 9.14.2 Titration Curve of a Buffer -- 9.14.3 Natural Buffer Solutions -- 9.14.4 Calculating Buffer pH -- 9.14.5 Buffer pH Calculation I -- 9.15 ChemTech Buffer Solution Calculator -- 9.16 Chapter Key Concepts -- 9.17 Chapter Problems -- 9.17.0 Ionization Reactions and Constants -- 9.17.0 Calculations with Ka and Kb -- 9.17.0 Solubility Product Ksp Calculations -- 9.17.0 Calculations Involving pH -- 9.17.0 Buffer Solution Calculations -- Chapter 10 Titration-A Volumetric Method of Analysis -- 10.1 Introduction -- 10.2 Reacting Ratios -- 10.3 The Equivalence Point -- 10.4 Useful Relationships for Calculations -- 10.5 Deriving the Titration Equation -- 10.5.1 Titration Calculation Example -- 10.6 Titrations in ChemTech -- 10.6.1 Acid/Base Titrations Using Molar Solutions -- 10.6.2 Titration Calculation Example -- 10.7 Acid/Base Titration Endpoint (Equivalence Point) -- 10.8 Acid/Base Titration Midpoint -- 10.9 Acid/Base Titration Indicators -- 10.9.1 The Ideal Indicator -- 10.10 Titrations Using Normal Solutions -- 10.10.1 Normal Solution Titration Example -- 10.11 Polyprotic Acid Titration -- 10.12 ChemTech Calculation of Normal Titrations -- 10.13 Performing a Titration -- 10.13.1 Titration Glassware -- 10.13.2 Titration Steps -- 10.14 Primary Standards -- 10.15 Standardization of Sodium Hydroxide -- 10.15.1 NaOH Titrant Standardization Example -- 10.16 Conductometric Titrations (Nonaqueous Solutions) -- 10.17 Precipitation Titration (MOHR Method for Halides) -- 10.17.1 Basic Steps in Titration -- 10.17.2 Important Considerations.
10.18 Complex Formation with Back Titration (Volhard Method for Anions).
Record Nr. UNINA-9910840545703321
Ham Bryan M  
Newark : , : John Wiley & Sons, Incorporated, , 2024
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Proteomics of biological systems [[electronic resource] ] : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham
Proteomics of biological systems [[electronic resource] ] : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham
Autore Ham Bryan M
Pubbl/distr/stampa Hoboken, N.J., : John Wiley & Sons, 2012
Descrizione fisica 1 online resource (376 p.)
Disciplina 572/.62
Soggetto topico Proteomics - Methodology
Phosphorylation - Research - Methodology
Phosphoproteins - Synthesis
Mass spectrometry
Biological systems - Research - Methodology
ISBN 1-283-28285-2
9786613282859
1-118-13703-5
1-118-13704-3
1-118-13701-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto PROTEOMICS OF BIOLOGICAL SYSTEMS: Protein Phosphorylation Using Mass Spectrometry Techniques; CONTENTS; PREFACE; ACKNOWLEDGMENTS; ABOUT THE AUTHOR; 1: Posttranslational Modification (PTM) of Proteins; 1.1 OVER 200 FORMS OF PTM OF PROTEINS; 1.2 THREE MAIN TYPES OF PTM STUDIED BY MS; 1.3 OVERVIEW OF NANO-ELECTROSPRAY/NANOFLOW LC-MS; 1.3.1 Definition and Description of MS; 1.3.2 Basic Design of Mass Analyzer Instrumentation; 1.3.3 ESI; 1.3.4 Nano-ESI; 1.4 OVERVIEW OF NUCLEIC ACIDS; 1.5 PROTEINS AND PROTEOMICS; 1.5.1 Introduction to Proteomics; 1.5.2 Protein Structure and Chemistry
1.5.3 Bottom-Up Proteomics: MS of Peptides1.5.3.1 History and Strategy; 1.5.3.2 Protein Identification through Product Ion Spectra; 1.5.3.3 High-Energy Product Ions; 1.5.3.4 De Novo Sequencing; 1.5.3.5 Electron Capture Dissociation (ECD); 1.5.4 Top-Down Proteomics: MS of Intact Proteins; 1.5.4.1 Background; 1.5.4.2 GP Basicity and Protein Charging; 1.5.4.3 Calculation of Charge State and Molecular Weight; 1.5.4.4 Top-Down Protein Sequencing; 1.5.5 Systems Biology and Bioinformatics; 1.5.6 Biomarkers in Cancer; REFERENCES; 2: Glycosylation of Proteins; 2.1 PRODUCTION OF A GLYCOPROTEIN
2.2 BIOLOGICAL PROCESSES OF PROTEIN GLYCOSYLATION2.3 N-LINKED AND O-LINKED GLYCOSYLATION; 2.4 CARBOHYDRATES; 2.4.1 Ionization of Oligosaccharides; 2.4.2 Carbohydrate Fragmentation; 2.4.3 Complex Oligosaccharide Structural Elucidation; 2.5 THREE OBJECTIVES IN STUDYING GLYCOPROTEINS; 2.6 GLYCOSYLATION STUDY APPROACHES; 2.6.1 MS of Glycopeptides; 2.6.2 Mass Pattern Recognition; 2.6.2.1 High Galactose Glycosylation Pattern; 2.6.3 Charge State Determination; 2.6.4 Diagnostic Fragment Ions; 2.6.5 High-Resolution/High-Mass Accuracy Measurement and Identification; 2.6.6 Digested Bovine Fetuin
REFERENCES3: Sulfation of Proteins as Posttranslational Modification; 3.1 GLYCOSAMINOGLYCAN SULFATION; 3.2 CELLULAR PROCESSES INVOLVED IN SULFATION; 3.3 BRIEF EXAMPLE OF PHOSPHORYLATION; 3.4 SULFOTRANSFERASE CLASS OF ENZYMES; 3.5 FRAGMENTATION NOMENCLATURE FOR CARBOHYDRATES; 3.6 SULFATED MUCIN OLIGOSACCHARIDES; 3.7 TYROSINE SULFATION; 3.8 TYROSYLPROTEIN SULFOTRANSFERASES TPST1 AND TPST2; 3.9 O-SULFATED HUMAN PROTEINS; 3.10 SULFATED PEPTIDE PRODUCT ION SPECTRA; 3.11 USE OF HIGHER ENERGY COLLISIONS; 3.12 ELECTRON CAPTURE DISSOCIATION (ECD); 3.13 SULFATION VERSUS PHOSPHORYLATION; REFERENCES
4: Eukaryote PTM as Phosphorylation: Normal State Studies4.1 MASS SPECTRAL MEASUREMENT WITH EXAMPLES OF HELA CELL PHOSPHOPROTEOME; 4.1.1 Introduction; 4.1.2 Protein Phosphatase and Kinase; 4.1.3 Hydroxy-Amino Acid Phosphorylation; 4.1.4 Traditional Phosphoproteomic Approaches; 4.1.5 Current Approaches; 4.1.5.1 Phosphoproteomic Enrichment Techniques; 4.1.5.2 IMAC; 4.1.5.3 MOAC; 4.1.5.4 Methylation of Peptides prior to IMAC or MOAC Enrichment; 4.1.6 The Ideal Approach; 4.1.7 One-Dimensional (1-D) Sodium Dodecyl Sulfate (SDS) PAGE; 4.1.8 Tandem MS Approach; 4.1.8.1 pS Loss of Phosphate Group
4.1.8.2 pT Loss of Phosphate Group
Record Nr. UNINA-9910139593603321
Ham Bryan M  
Hoboken, N.J., : John Wiley & Sons, 2012
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui
Proteomics of biological systems [[electronic resource] ] : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham
Proteomics of biological systems [[electronic resource] ] : protein phosphorylation using mass spectrometry techniques / / Bryan M Ham
Autore Ham Bryan M
Pubbl/distr/stampa Hoboken, N.J., : John Wiley & Sons, 2012
Descrizione fisica 1 online resource (376 p.)
Disciplina 572/.62
Soggetto topico Proteomics - Methodology
Phosphorylation - Research - Methodology
Phosphoproteins - Synthesis
Mass spectrometry
Biological systems - Research - Methodology
ISBN 1-283-28285-2
9786613282859
1-118-13703-5
1-118-13704-3
1-118-13701-9
Formato Materiale a stampa
Livello bibliografico Monografia
Lingua di pubblicazione eng
Nota di contenuto PROTEOMICS OF BIOLOGICAL SYSTEMS: Protein Phosphorylation Using Mass Spectrometry Techniques; CONTENTS; PREFACE; ACKNOWLEDGMENTS; ABOUT THE AUTHOR; 1: Posttranslational Modification (PTM) of Proteins; 1.1 OVER 200 FORMS OF PTM OF PROTEINS; 1.2 THREE MAIN TYPES OF PTM STUDIED BY MS; 1.3 OVERVIEW OF NANO-ELECTROSPRAY/NANOFLOW LC-MS; 1.3.1 Definition and Description of MS; 1.3.2 Basic Design of Mass Analyzer Instrumentation; 1.3.3 ESI; 1.3.4 Nano-ESI; 1.4 OVERVIEW OF NUCLEIC ACIDS; 1.5 PROTEINS AND PROTEOMICS; 1.5.1 Introduction to Proteomics; 1.5.2 Protein Structure and Chemistry
1.5.3 Bottom-Up Proteomics: MS of Peptides1.5.3.1 History and Strategy; 1.5.3.2 Protein Identification through Product Ion Spectra; 1.5.3.3 High-Energy Product Ions; 1.5.3.4 De Novo Sequencing; 1.5.3.5 Electron Capture Dissociation (ECD); 1.5.4 Top-Down Proteomics: MS of Intact Proteins; 1.5.4.1 Background; 1.5.4.2 GP Basicity and Protein Charging; 1.5.4.3 Calculation of Charge State and Molecular Weight; 1.5.4.4 Top-Down Protein Sequencing; 1.5.5 Systems Biology and Bioinformatics; 1.5.6 Biomarkers in Cancer; REFERENCES; 2: Glycosylation of Proteins; 2.1 PRODUCTION OF A GLYCOPROTEIN
2.2 BIOLOGICAL PROCESSES OF PROTEIN GLYCOSYLATION2.3 N-LINKED AND O-LINKED GLYCOSYLATION; 2.4 CARBOHYDRATES; 2.4.1 Ionization of Oligosaccharides; 2.4.2 Carbohydrate Fragmentation; 2.4.3 Complex Oligosaccharide Structural Elucidation; 2.5 THREE OBJECTIVES IN STUDYING GLYCOPROTEINS; 2.6 GLYCOSYLATION STUDY APPROACHES; 2.6.1 MS of Glycopeptides; 2.6.2 Mass Pattern Recognition; 2.6.2.1 High Galactose Glycosylation Pattern; 2.6.3 Charge State Determination; 2.6.4 Diagnostic Fragment Ions; 2.6.5 High-Resolution/High-Mass Accuracy Measurement and Identification; 2.6.6 Digested Bovine Fetuin
REFERENCES3: Sulfation of Proteins as Posttranslational Modification; 3.1 GLYCOSAMINOGLYCAN SULFATION; 3.2 CELLULAR PROCESSES INVOLVED IN SULFATION; 3.3 BRIEF EXAMPLE OF PHOSPHORYLATION; 3.4 SULFOTRANSFERASE CLASS OF ENZYMES; 3.5 FRAGMENTATION NOMENCLATURE FOR CARBOHYDRATES; 3.6 SULFATED MUCIN OLIGOSACCHARIDES; 3.7 TYROSINE SULFATION; 3.8 TYROSYLPROTEIN SULFOTRANSFERASES TPST1 AND TPST2; 3.9 O-SULFATED HUMAN PROTEINS; 3.10 SULFATED PEPTIDE PRODUCT ION SPECTRA; 3.11 USE OF HIGHER ENERGY COLLISIONS; 3.12 ELECTRON CAPTURE DISSOCIATION (ECD); 3.13 SULFATION VERSUS PHOSPHORYLATION; REFERENCES
4: Eukaryote PTM as Phosphorylation: Normal State Studies4.1 MASS SPECTRAL MEASUREMENT WITH EXAMPLES OF HELA CELL PHOSPHOPROTEOME; 4.1.1 Introduction; 4.1.2 Protein Phosphatase and Kinase; 4.1.3 Hydroxy-Amino Acid Phosphorylation; 4.1.4 Traditional Phosphoproteomic Approaches; 4.1.5 Current Approaches; 4.1.5.1 Phosphoproteomic Enrichment Techniques; 4.1.5.2 IMAC; 4.1.5.3 MOAC; 4.1.5.4 Methylation of Peptides prior to IMAC or MOAC Enrichment; 4.1.6 The Ideal Approach; 4.1.7 One-Dimensional (1-D) Sodium Dodecyl Sulfate (SDS) PAGE; 4.1.8 Tandem MS Approach; 4.1.8.1 pS Loss of Phosphate Group
4.1.8.2 pT Loss of Phosphate Group
Record Nr. UNINA-9910821480603321
Ham Bryan M  
Hoboken, N.J., : John Wiley & Sons, 2012
Materiale a stampa
Lo trovi qui: Univ. Federico II
Opac: Controlla la disponibilità qui